We developed an efficient "Combinatorial Strategy” for cloning different vectors with various clone sites. 1) Using originally existed clone sites from circular vectors to prepare the inserts, and if ...

While the traditional cloning workflow has been used in laboratories for decades, new techniques have emerged that allow for rapid assembly of multiple genetic fragments. The Electra Vector System, ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...

Vol. 1: Ch. 1. Plasmids and their usefulness in molecular cloning -- Ch. 2. Bacteriophage and its vectors -- Ch. 3. Working with bacteriophage M13 vectors -- Ch. 4. Working with high-capacity vectors ...

Nereus Gunther is a molecular biologist at the Agricultural Research Service within the US Department of Agriculture (Washington D.C., USA), where part of his research focuses on Campylobacter and its ...